Differences in economic, cultural, social and educational factors between urban and rural areas in Canada might influence behavioural and mental health outcomes. However, most research assessing differences in urban and rural patterns of substance use has been conducted in the United States. Within Canada, information is limited on whether students who attend urban and rural schools differ in substance use. To address this gap, this report analyzed data from regularly occurring student surveys and outlines differences and similarities between students who attend urban and rural schools. Though this report is technical in nature, it has implications for a broader audience such as those working in prevention programming and schools, and others who work with youth. Understanding differences in Canada between adolescent students in urban and rural communities can help guide best practices in prevention programs for schools and communities.